J Biotechnol. 2004 Aug 26;112(1-2):5-12.

Single molecule force spectroscopy on ligand-DNA complexes: from molecular binding mechanisms to biosensor applications.

Ros R, Eckel R, Bartels F, Sischka A, Baumgarth B, Wilking SD, Pühler A, Sewald N, Becker A, Anselmetti D.

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Experimental Biophysics, Faculty of Physics, Bielefeld University, Universitätsstrasse 25, 33615 Bielefeld, Germany. robert.ross@physik.uni-bielefeld.de

Abstract

Recent developments in single molecule force spectroscopy (SMFS) allow direct observation and measurements of forces that hold protein-DNA complexes together. Furthermore, the mechanics of double-stranded (ds) DNA molecules in the presence of small binding ligands can be detected. The results elucidate molecular binding mechanisms and open the way for ultra sensitive and powerful biosensor applications.

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