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    Nature. 2004 Jul 29;430(6999):578-82.

    Structural determinants for generating centromeric chromatin.

    Black BE, Foltz DR, Chakravarthy S, Luger K, Woods VL Jr, Cleveland DW.

    Source

    Ludwig Institute for Cancer Research, University of California, San Diego, La Jolla, California 92093, USA.

    Abstract

    Mammalian centromeres are not defined by a consensus DNA sequence. In all eukaryotes a hallmark of functional centromeres--both normal ones and those formed aberrantly at atypical loci--is the accumulation of centromere protein A (CENP-A), a histone variant that replaces H3 in centromeric nucleosomes. Here we show using deuterium exchange/mass spectrometry coupled with hydrodynamic measures that CENP-A and histone H4 form sub-nucleosomal tetramers that are more compact and conformationally more rigid than the corresponding tetramers of histones H3 and H4. Substitution into histone H3 of the domain of CENP-A responsible for compaction is sufficient to direct it to centromeres. Thus, the centromere-targeting domain of CENP-A confers a unique structural rigidity to the nucleosomes into which it assembles, and is likely to have a role in maintaining centromere identity.

    PMID:
    15282608
    [PubMed - indexed for MEDLINE]

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