Warning: The NCBI web site requires JavaScript to function. more...
Generate a file for use with external citation management software.
Southeast Collaboratory for Structural Genomics, A222 Life Sciences, Athens, GA 30602-7229, USA. mmayer@secsg.org
The human protein production group at the Southeast Collaboratory for Structural Genomics is charged with producing human proteins for both X-ray crystallography and NMR structural studies. Eukaryotic, and human proteins in particular, are notoriously difficult to express in bacterial systems. For various reasons, T7-based expression often results in protein expressed in an insoluble form. Overcoming this requires either introduction of a step to screen expression conditions or inclusion of a troublesome refolding step during purification. Our laboratory uses a trc-based expression vector that addresses many of the difficulties of the commonly used T7-based expression systems. Proteins expressed under the trc promoter, a weak promoter compared to the strong T7 promoter, are produced in a soluble form and include necessary cofactors. The details of this system will be discussed.
Your browsing activity is empty.
Activity recording is turned off.
Turn recording back on