Mol Cell. 2004 Jul 23;15(2):303-13.

Genomic run-on evaluates transcription rates for all yeast genes and identifies gene regulatory mechanisms.

García-Martínez J, Aranda A, Pérez-Ortín JE.

Source

Departamento de Bioquímica y Biología Molecular, Universitat de València, Burjassot, Spain.

Abstract

Most studies of eukaryotic gene regulation have been done looking at mature mRNA levels. Nevertheless, the steady-state mRNA level is the result of two opposing factors: transcription rate (TR) and mRNA degradation. Both can be important points to regulate gene expression. Here we show a new method that combines the use of nylon macroarrays and in vivo radioactive labeling of nascent RNA to quantify TRs, mRNA levels, and mRNA stabilities for all the S. cerevisiae genes. We found that during the shift from glucose to galactose, most genes undergo drastic changes in TR and mRNA stability. However, changes in mRNA levels are less pronounced. Some genes, such as those encoding mitochondrial proteins, are coordinately regulated in mRNA stability behaving as decay regulons. These results indicate that, although TR is the main determinant of mRNA abundance in yeast, modulation of mRNA stability is a key factor for gene regulation.

PMID:: 15260981; [PubMed - indexed for MEDLINE]

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Research Support, Non-U.S. Gov't

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GENBANK/GSM15282
GENBANK/GSM15284
GENBANK/GSM15285
GENBANK/GSM15286
GENBANK/GSM15287
GENBANK/GSM15288
GENBANK/GSM15289
GENBANK/GSM15290
GENBANK/GSM15291
GENBANK/GSM15292
GENBANK/GSM15293
GENBANK/GSM15294
GENBANK/GSM15295
GENBANK/GSM15296
GENBANK/GSM15297
GENBANK/GSM15298
GENBANK/GSM15299
GENBANK/GSM15300
GENBANK/GSM15301
GENBANK/GSM15302
GENBANK/GSM15303
GENBANK/GSM15304
GENBANK/GSM15305
GENBANK/GSM15306
GENBANK/GSM15307
GENBANK/GSM15308
GENBANK/GSM15310
GENBANK/GSM15311
GENBANK/GSM15313
GENBANK/GSM15315
GENBANK/GSM15317
GENBANK/GSM15319
GENBANK/GSM15322
GENBANK/GSM15324
GENBANK/GSM15326
GENBANK/GSM15328
GENBANK/GSM15341
GENBANK/GSM15343
GENBANK/GSM15345
GENBANK/GSM15347
GENBANK/GSM15351
GENBANK/GSM15352

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