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FEBS Lett. 2004 Jul 16;570(1-3):102-6.

Characterization of H2O2-induced acute apoptosis in cultured neural stem/progenitor cells.

Author information

  • 1Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC 20375, USA. jlin@cbmse.nrl.navy.mil

Abstract

In the present study, we characterized hydrogen peroxide (H2O2)-induced cell apoptosis and related cell signaling pathways in cultured embryonic neural stem/progenitor cells (NS/PCs). Our data indicated that H2O2 induced acute cell apoptosis in NS/PC in concentration- and time-dependent manners and selectively, it transiently increased PI3K-Akt and Mek-Erk1/2 in a dose-dependent manner. Inhibition of PI3K-Akt with wortmannin, a PI3-K inhibitor, was found to significantly increase H2O2-induced acute apoptosis and dramatically decrease basal pGSK3beta levels. The level of pGSK3beta remained unchanged with H2O2 exposure. We conclude that the transient activation of PI3K-Akt signaling delays the H2O2-induced acute apoptosis in cultured NS/PCs in part through maintaining the basal pGSK3beta level and activating other downstream effectors.

PMID:
15251448
[PubMed - indexed for MEDLINE]
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