Yi Chuan Xue Bao. 1992;19(2):117-21.

[Identification of sex in mouse preimplantation embryos by indirect immunofluorescent assay].

[Article in Chinese]

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Kunming Institute of Zoology, Academia Sinica.

Abstract

Indirect immunofluorescent assay was used for detecting male-specific H-Y antigen on preimplantation embryos. Our results showed that, embryos examined, 54.1% were H-Y positive (male) and 45.9% were H-Y negative (female). Significant difference was not found between natural sex ratio of kunming strain mouse (male: 52.1%; female: 47.9%) and our results (P greater than 0.05). Moreover, the results sequentially were confirmed by cytogenetic method. 75.50% H-Y positive embryos were male and 82.97% H-Y negative embryos were female respectively (P less than 0.01). Some factors influencing the accurency of sex selection with indirect immunofluorescent assay were also discussed.

PMID:: 1524843; [PubMed - indexed for MEDLINE]

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[Identification of sex in mouse preimplantation embryos by indirect immunofluorescent assay].

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