Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
Trends Biotechnol. 2004 Jul;22(7):346-53.

Codon bias and heterologous protein expression.

Author information

  • 1DNA 2.0, 1455 Adams Drive, Menlo Park, CA 94025, USA. cgustafsson@dnatwopointo.com


The expression of functional proteins in heterologous hosts is a cornerstone of modern biotechnology. Unfortunately, proteins are often difficult to express outside their original context. They might contain codons that are rarely used in the desired host, come from organisms that use non-canonical code or contain expression-limiting regulatory elements within their coding sequence. Improvements in the speed and cost of gene synthesis have facilitated the complete redesign of entire gene sequences to maximize the likelihood of high protein expression. Redesign strategies are discussed here, including modification of translation initiation regions, alteration of mRNA structural elements and use of different codon biases.

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk