Institut des Sciences de la Vie, Université Catholique de Louvain, Croix du Sud 2-20, 1348 Louvain-la-Neuve, Belgium.
We assess five years of usage of the major genome-wide collections of mutants from Saccharomyces cerevisiae: single deletion mutants, double mutants conferring 'synthetic' lethality and the 'TRIPLES' collection of mutants obtained by random transposon insertion. Over 100 experimental conditions have been tested and more than 5,000 novel phenotypic traits have been assigned to yeast genes using these collections.