Interactions of PIP1 with TIN2 and POT1 and localization of PIP1 to telomeres. (A) Co-IP of PIP1 with POT1 and TIN2. Epitope-tagged constructs were transfected into 293T cells in various combinations (top) and subjected to coIP using antibodies to the myc or HA tags. Protein expression levels are shown in input lysates. Immunoblots were done with the myc antibody to detect POT1 and the Flag antibody to detect PIP1 and TIN2. (B) Co-IP of PIP1 with TIN2 but not TRF1. The indicated constructs were cotransfected into 293T cells, and the anti-myc (PIP1) IPs were analyzed for the presence of PIP1 (myc antibody), TRF1, and TIN2 (Flag antibody for both). (C) PIP1 does not interact with TIN2-13. N-FH2-TIN2 or N-FH2-TIN2-13 were coexpressed with Flag-PIP1 and myc-TRF1 as indicated. Proteins recovered in the anti-HA immunoprecipitate were analyzed by immunoblotting. TRF1 was detected with the myc antibody, and PIP1, TIN2, and TIN2-13 were detected with the Flag antibody. (D) PIP1 interacts with POT1ΔOB. Flag-PIP1 was coexpressed with myc-tagged full-length POT1 or POT1ΔOB. IPs were performed with Flag-antibody and analyzed by immunoblotting with antibodies to the epitope tags. (E) Telomeric localization of exogenous PIP1. IF of HeLa1.2.11 cells expressing Flag-PIP1 from a retrovirus (pLPC). PIP1 was detected with anti-Flag (M2; green). TRF1 (top) and TIN2 (bottom) were detected with Abs 371 and 865, respectively (red). DNA was stained with DAPI (shown in the merged panel).

Telomere elongation induced by RNAi-mediated reduction of POT1. (A) Immunoblot of HTC75 cells expressing the indicated shRNAs (vector control represents cells infected with the empty virus) at PD50 postselection. POT1 was detected with Ab 978 as described previously (Loayza and de Lange 2003). The asterisk marks a cross-reacting band that serves as a loading control. (B) Telomeric restriction fragment blot of the cell lines shown in A performed at the indicated population doublings (PDs). The molecular mass in kilobases of HindIII-digested λ DNA fragments is shown on the left. (C) Graph of the mean telomeric restriction fragment length of the indicated cell lines plotted versus PD. Elongation rates of the telomeres are indicated to the right.

Telomere elongation induced by RNAi-mediated reduction of PIP1. (A) HTC75 cells expressing myc-tagged PIP1 were transfected with siRNA targeting luciferase (control) and two independent siRNAs targeting PIP1 (#3 and #4). Myc-PIP1 levels were detected by immunoblotting using 9E10 (Oncogene) with α-tubulin as loading control. (B) Telomere lengthening caused by PIP1 knockdown. Telomeric restriction fragment blot of the HTC75 cells infected with PIP1 shRNA retroviruses or Flag-tagged PIP1. Population doublings (PDs) are indicated above the lanes. The molecular mass in kilobases of HindIII-digested λ DNA fragments is shown on the left. (C) Graph of the mean telomeric restriction fragment length of the indicated cell lines plotted versus PD. Elongation rates of the telomeres are indicated to the right.

Summary of the TRF1-TIN2-PIP1-POT1 telomere length regulation pathway. (A) Components of the TRF1 complex and their interactions with DNA and/or proteins. The PIP1 interaction with TIN2 has not been mapped in detail but is abolished by deletion of the indicated domain. (B) Schematic of POT1-mediated telomere length regulation, emphasizing the protein interactions leading to recruitment of POT1. It is possible (likely) that some or all of the components of the TRF1 complex are associated with each other prior to the binding of TRF1 to telomeric DNA. It is not known whether POT1 can bind to single-stranded DNA while interacting with PIP1.