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Proc Natl Acad Sci U S A. 2004 Jul 6;101(27):9955-9. Epub 2004 Jun 28.

Labeling of fusion proteins with synthetic fluorophores in live cells.

Author information

  • 1Institute of Chemical Sciences and Engineering, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland.

Abstract

A general approach for the sequential labeling of fusion proteins of O(6)-alkylguanine-DNA alkyltransferase (AGT) with different fluorophores in mammalian cells is presented. AGT fusion proteins with different localizations in the cell can be labeled specifically with different fluorophores, and the fluorescence labeling can be used for applications such as multicolor analysis of dynamic processes and fluorescence resonance energy transfer measurements. The facile access to a variety of different AGT substrates as well as the specificity of the labeling reaction should make the approach an important tool to study protein function in live cells.

PMID:
15226507
[PubMed - indexed for MEDLINE]
PMCID:
PMC454197
Free PMC Article

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