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1: Bioorg Med Chem Lett. 2004 Aug 2;14(15):4001-4.Click here to read Links

Aptamer selection for the inhibition of cell adhesion with fibronectin as target.

Ogawa A, Tomita N, Kikuchi N, Sando S, Aoyama Y.

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-Ku, Kyoto 615-8510, Japan.

An affinity column immobilizing a decapeptide H(2)N-RGDSPASSKP-CO(2)H was used to select RGD-binding aptamers from a pool of 86-mer single-strand oligodeoxynucleotides (ODNs) containing a random 40-mer sequence. The enriched library thus obtained was further selected against adsorbed fibronectin and individual aptamers were monocloned in E. coli and sequenced to give a couple of highly homologous ODNs, which indeed inhibited fibronectin-integrin mediated cell adhesion.

PMID: 15225715 [PubMed - indexed for MEDLINE]

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