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1: Genomics. 2004 Jul;84(1):205-10.Click here to read Links

Circular rapid amplification of cDNA ends for high-throughput extension cloning of partial genes.

Fu GK, Wang JT, Yang J, Au-Young J, Stuve LL.

Incyte Corporation, Palo Alto, CA 94304, USA. gfu@incyte.com

The rapid amplification of cDNA ends (RACE) procedure is a widely used PCR-based method to clone the cDNA ends of mRNA transcripts. Current RACE methods often produce a high background of nonspecific PCR products, which can exclude the identification of the target cDNA of interest. We describe here an improved RACE procedure using circular cDNA templates and demonstrate the successful extension cloning of 4406 cDNAs. Copyright 2004 Elsevier Inc.

PMID: 15203218 [PubMed - indexed for MEDLINE]