Electrophoresis. 2004 Jun;25(10-11):1496-502.

Simultaneous determination of polyamines and catecholamines in PC-12 tumor cell extracts by capillary electrophoresis with laser-induced fluorescence detection.

Source

Department of Chemistry and Center for Environmental Science & Technology, University of Missouri-Rolla, 1870 Miner Circle, Rolla, MO 65409, USA.

Abstract

A method to detect polyamines and catecholamines in PC-12 tumor cell extracts by capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is described for the first time. Both derivatization conditions and buffer concentrations and pH were optimized. Under optimized conditions the polyamines (putresine, spermine, spermidine) and catecholamines (dopamine, norepinephrine, epinephrine, serotonin) were derivatized with fluorescein isothiocyanate and separated at 25 kV in a fused-silica capillary (50 microm ID x 40 cm) with 0.1 M borate, pH 9.0, in less than 18 min. The influence of running buffer conditions, such as buffer pH and concentrations, were also investigated. Linearity of the analytes ranged from 0.05 to 1.0 micromol/L, and the detection limit (S/N = 3 ) ranged from 0.03 to 2.50 nmol/L. The concentrations of polyamines and catecholamines in PC-12 tumor cell extracts were determined with this method.

PMID:: 15188232; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Supplemental Content

Save items

Related citations in PubMed

Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection. [J Chromatogr B Analyt Technol ...]
Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection.
Liu G, Chen J, Ma Y. J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jun 15; 805(2):281-8.
Direct tris(2,2'-bipyridyl)ruthenium (II) electrochemiluminescence detection of polyamines separated by capillary electrophoresis. [Electrophoresis. 2003]
Direct tris(2,2'-bipyridyl)ruthenium (II) electrochemiluminescence detection of polyamines separated by capillary electrophoresis.
Liu J, Yang X, Wang E. Electrophoresis. 2003 Sep; 24(18):3131-8.
Simultaneous determination of histamine and polyamines by capillary zone electrophoresis with 4-fluor-7-nitro-2,1,3-benzoxadiazole derivatization and fluorescence detection. [J Chromatogr B Analyt Technol ...]
Simultaneous determination of histamine and polyamines by capillary zone electrophoresis with 4-fluor-7-nitro-2,1,3-benzoxadiazole derivatization and fluorescence detection.
Zhang LY, Tang XC, Sun MX. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Jun 25; 820(2):211-9.
Review Characterization of proteins by capillary electrophoresis in fused-silica columns: review on serum protein analysis and application to immunoassays. [Electrophoresis. 1994]
Review Characterization of proteins by capillary electrophoresis in fused-silica columns: review on serum protein analysis and application to immunoassays.
Chen FT, Sternberg JC. Electrophoresis. 1994 Jan; 15(1):13-21.
Review Microdialysis monitoring of catecholamines and excitatory amino acids in the rat and mouse brain: recent developments based on capillary electrophoresis with laser-induced fluorescence detection--a mini-review. [Cell Mol Neurobiol. 2003]
Review Microdialysis monitoring of catecholamines and excitatory amino acids in the rat and mouse brain: recent developments based on capillary electrophoresis with laser-induced fluorescence detection--a mini-review.
Parrot S, Bert L, Mouly-Badina L, Sauvinet V, Colussi-Mas J, Lambás-Señas L, Robert F, Bouilloux JP, Suaud-Chagny MF, Denoroy L, et al. Cell Mol Neurobiol. 2003 Oct; 23(4-5):793-804.

See reviews... See all...

Cited by 1 PubMed Central article

Involvement of potD in Streptococcus pneumoniae polyamine transport and pathogenesis. [Infect Immun. 2006]
Involvement of potD in Streptococcus pneumoniae polyamine transport and pathogenesis.
Ware D, Jiang Y, Lin W, Swiatlo E. Infect Immun. 2006 Jan; 74(1):352-61.

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Cited in PMC
Full-text articles in the PubMed Central Database that cite the current articles.

Recent activity

Clear Turn Off Turn On

Simultaneous determination of polyamines and catecholamines in PC-12 tumor cell ...
Simultaneous determination of polyamines and catecholamines in PC-12 tumor cell extracts by capillary electrophoresis with laser-induced fluorescence detection.
Electrophoresis. 2004 Jun ;25(10-11):1496-502.

PubMed
Elevated sister chromatid exchange phenotype of Bloom syndrome cells is compleme...
Elevated sister chromatid exchange phenotype of Bloom syndrome cells is complemented by human chromosome 15.
Proc Natl Acad Sci U S A. 1992 Sep 1 ;89(17):7968-72.

PubMed
Inflammatory neuropathies of the enteric nervous system.
Inflammatory neuropathies of the enteric nervous system.
Gastroenterology. 2004 Jun ;126(7):1872-83.

PubMed
Urothelial carcinoma of the urinary bladder with a component of acinar/tubular t...
Urothelial carcinoma of the urinary bladder with a component of acinar/tubular type differentiation simulating prostatic adenocarcinoma.
Hum Pathol. 2004 Jun ;35(6):769-73.

PubMed
Hagfish humoral defense protein exhibits structural and functional homology with...
Hagfish humoral defense protein exhibits structural and functional homology with mammalian complement components.
Proc Natl Acad Sci U S A. 1992 Sep 1 ;89(17):7910-4.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: