J Microbiol Methods. 2004 Jul;58(1):139-42.

Improved method for the isolation of RNA from (standing liquid cultures of) Streptomycetes.

van Keulen G, Siebring J, Rembacz KP, Hoogeveen M, Tomczynska M, Dijkhuizen L.

Source

Department of Microbiology, Groningen Biotechnology and Biomolecular Science Institute (GBB), University of Groningen, PO Box 14, 9750 AA Haren, The Netherlands. g.van.Keulen@biol.rug.nl

Abstract

Streptomycetes are complex soil bacteria capable of producing aerial reproductive mycelium and secondary metabolites. We observed novel phenomena such as an extended life cycle including flotation and anaerobiosis using standing liquid cultures. This paper describes an improved method for isolating good quality RNA from standing liquid cultures of S. coelicolor via excellent cell lysis.

PMID:: 15177913; [PubMed - indexed for MEDLINE]

Publication Types, MeSH Terms, Substances

Publication Types

MeSH Terms

Substances

LinkOut - more resources

Full Text Sources

Other Literature Sources

COS Scholar Universe

Molecular Biology Databases

PHENOL - HSDB

Supplemental Content

Save items

Related citations in PubMed

Isolation of high quality RNA from Streptomyces. [J Microbiol Methods. 2004]
Isolation of high quality RNA from Streptomyces.
Van Dessel W, Van Mellaert L, Geukens N, Lammertyn E, Anné J. J Microbiol Methods. 2004 Jul; 58(1):135-7.
Differentiation and anaerobiosis in standing liquid cultures of Streptomyces coelicolor. [J Bacteriol. 2003]
Differentiation and anaerobiosis in standing liquid cultures of Streptomyces coelicolor.
van Keulen G, Jonkers HM, Claessen D, Dijkhuizen L, Wösten HA. J Bacteriol. 2003 Feb; 185(4):1455-8.
Review The use of the rare UUA codon to define "expression space" for genes involved in secondary metabolism, development and environmental adaptation in streptomyces. [J Microbiol. 2008]
Review The use of the rare UUA codon to define "expression space" for genes involved in secondary metabolism, development and environmental adaptation in streptomyces.
Chater KF, Chandra G. J Microbiol. 2008 Feb; 46(1):1-11.
Aerial hyphae in surface cultures of Streptomyces lividans and Streptomyces coelicolor originate from viable segments surviving an early programmed cell death event. [FEMS Microbiol Lett. 2007]
Aerial hyphae in surface cultures of Streptomyces lividans and Streptomyces coelicolor originate from viable segments surviving an early programmed cell death event.
Manteca A, Claessen D, Lopez-Iglesias C, Sanchez J. FEMS Microbiol Lett. 2007 Sep; 274(1):118-25.
Review Signalling early developmental events in two highly diverged Streptomyces species. [Mol Microbiol. 2003]
Review Signalling early developmental events in two highly diverged Streptomyces species.
Chater KF, Horinouchi S. Mol Microbiol. 2003 Apr; 48(1):9-15.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.

Recent activity

Clear Turn Off Turn On

Improved method for the isolation of RNA from (standing liquid cultures of) Stre...
Improved method for the isolation of RNA from (standing liquid cultures of) Streptomycetes.
J Microbiol Methods. 2004 Jul ;58(1):139-42.

PubMed
Cystic fibrosis newborn screening: shifting the key question from "should we scr...
Cystic fibrosis newborn screening: shifting the key question from "should we screen?" to "how should we screen?".
Pediatrics. 2004 Jun ;113(6):1811-2.

PubMed
15173512[uid] (1)
PubMed
Roles of MITF for development of mast cells in mice: effects on both precursors ...
Roles of MITF for development of mast cells in mice: effects on both precursors and tissue environments.
Blood. 2004 Sep 15 ;104(6):1656-61. Epub 2004 Jun 1 .

PubMed
15022129[uid] (1)
PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Result Filters

Display Settings:

Send to: