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Retrovirology. 2004 Apr 5;1:5.

A murine leukemia virus with Cre-LoxP excisible coding sequences allowing superinfection, transgene delivery, and generation of host genomic deletions.

Author information

  • 1Department of Microbiology and Immunology, University of California, San Francisco 94143-0414, USA. cliffw@itsa.ucsf.edu

Abstract

BACKGROUND:

To generate a replication-competent retrovirus that could be conditionally inactivated, we flanked the viral genes of the Akv murine leukemia virus with LoxP sites. This provirus can delete its envelope gene by LoxP/Cre mediated recombination and thereby allow superinfection of Cre recombinase expressing cells.

RESULTS:

In our studies, the virus repeatedly infected the cell and delivered multiple copies of the viral genome to the host genome; the superinfected cells expressed a viral transgene on average twenty times more than non-superinfected cells. The insertion of multiple LoxP sites into the cellular genome also led to genomic deletions, as demonstrated by comparative genome hybridization.

CONCLUSION:

We envision that this technology may be particularly valuable for delivering transgenes and/or causing deletions.

PMID:
15169571
[PubMed - indexed for MEDLINE]
PMCID:
PMC419723
Free PMC Article

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