Derivatization of amino acids with N,N-dimethyl-2,4-dinitro-5-fluorobenzylamine for liquid chromatography/electrospray ionization mass spectrometry

Rapid Commun Mass Spectrom. 2004;18(10):1059-65. doi: 10.1002/rcm.1443.

Abstract

Derivatization, separation and identification of amino acids with a novel compound, N,N-dimethyl-2,4-dinitro-5-fluorobenzylamine (DMDNFB), using high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) was demonstrated. Compared to derivatization with 2,4-dinitrofluorobenzene (DNFB), DMDNFB-derivatized amino acids and dipeptides exhibit much larger ion current signals in the commonly used ESI positive mode, which was attributed to the introduction of the N,N-dimethylaminomethyl protonatable site.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acids / analysis*
  • Amino Acids / chemistry*
  • Benzylamines
  • Chromatography, Agarose / methods
  • Chromatography, High Pressure Liquid / methods
  • Dinitrofluorobenzene
  • Indicators and Reagents
  • Sensitivity and Specificity
  • Sepharose / analogs & derivatives*
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Spectrometry, Mass, Electrospray Ionization / statistics & numerical data

Substances

  • Amino Acids
  • Benzylamines
  • Indicators and Reagents
  • phenyl-sepharose
  • Sepharose
  • Dinitrofluorobenzene