Abstract
Derivatization, separation and identification of amino acids with a novel compound, N,N-dimethyl-2,4-dinitro-5-fluorobenzylamine (DMDNFB), using high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC/ESI-MS) was demonstrated. Compared to derivatization with 2,4-dinitrofluorobenzene (DNFB), DMDNFB-derivatized amino acids and dipeptides exhibit much larger ion current signals in the commonly used ESI positive mode, which was attributed to the introduction of the N,N-dimethylaminomethyl protonatable site.
Copyright 2004 John Wiley & Sons, Ltd.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acids / analysis*
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Amino Acids / chemistry*
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Benzylamines
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Chromatography, Agarose / methods
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Chromatography, High Pressure Liquid / methods
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Dinitrofluorobenzene
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Indicators and Reagents
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Sensitivity and Specificity
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Sepharose / analogs & derivatives*
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Spectrometry, Mass, Electrospray Ionization / methods*
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Spectrometry, Mass, Electrospray Ionization / statistics & numerical data
Substances
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Amino Acids
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Benzylamines
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Indicators and Reagents
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phenyl-sepharose
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Sepharose
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Dinitrofluorobenzene