CcpA-dependent regulation of Bacillus subtilis glutamate dehydrogenase gene expression

J Bacteriol. 2004 Jun;186(11):3392-8. doi: 10.1128/JB.186.11.3392-3398.2004.

Abstract

The Bacillus subtilis rocG gene, encoding catabolic glutamate dehydrogenase, was found to be subject to direct CcpA-dependent glucose repression. The effect of CcpA required the presence of both the HPr and Crh proteins. The primary CcpA binding site was identified by mutational analysis and DNase I footprinting. In the absence of inducers of the Roc pathway, rocG was still expressed at a low level due to readthrough transcription. CcpA-dependent repression of rocG readthrough transcription proved to contribute to the slow growth rate of B. subtilis cells in glucose-glutamate medium. Increased readthrough expression of rocG was shown to be partially responsible for the growth defect of ccpA strains in glucose-ammonium medium.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Bacillus subtilis / enzymology*
  • Bacillus subtilis / genetics
  • Base Sequence
  • Gene Expression Regulation, Bacterial*
  • Glucose / pharmacology
  • Glutamate Dehydrogenase / genetics*
  • Glutamic Acid / metabolism
  • Molecular Sequence Data
  • Ornithine / pharmacology
  • Promoter Regions, Genetic

Substances

  • Glutamic Acid
  • Ornithine
  • Glutamate Dehydrogenase
  • Glucose