The phosphoprotein StarD10 is overexpressed in breast cancer and cooperates with ErbB receptors in cellular transformation

Monilola A Olayioye; Peter Hoffmann; Thomas Pomorski; Jane Armes; Richard J Simpson; Bruce E Kemp; Geoffrey J Lindeman; Jane E Visvader

doi:10.1158/0008-5472.CAN-03-3731

The phosphoprotein StarD10 is overexpressed in breast cancer and cooperates with ErbB receptors in cellular transformation

Cancer Res. 2004 May 15;64(10):3538-44. doi: 10.1158/0008-5472.CAN-03-3731.

Authors

Monilola A Olayioye¹, Peter Hoffmann, Thomas Pomorski, Jane Armes, Richard J Simpson, Bruce E Kemp, Geoffrey J Lindeman, Jane E Visvader

Affiliation

¹ The Walter and Eliza Hall Institute of Medical Research and Bone Marrow Research Laboratories, Royal Melbourne Hospital, Parkville, Victoria, Australia.

PMID: 15150109
DOI: 10.1158/0008-5472.CAN-03-3731

Abstract

We have identified that StarD10, a member of the START protein family, is overexpressed in both mouse and human breast tumors. StarD10 was initially discovered on the basis of its cross-reactivity with a phosphoserine-specific antibody in mammary tumors from Neu/ErbB2 transgenic mice and subsequently isolated from SKBR3 human breast carcinoma cells using a multistep biochemical purification strategy. We have shown that StarD10 is capable of binding lipids. StarD10 was found to be overexpressed in 35% of primary breast carcinomas and 64% of human breast cancer cell lines, correlating with their ErbB2/Her2 status. Coexpression of StarD10 with ErbB1/epidermal growth factor receptor in murine fibroblasts enhanced anchorage-independent growth in soft agar, providing evidence for functional cooperation between StarD10 and ErbB receptor signaling. Taken together, these data suggest that overexpression of this lipid-binding protein contributes to breast oncogenesis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Antibody Specificity
Breast Neoplasms / metabolism*
Breast Neoplasms / pathology
Cell Line, Tumor
Cell Transformation, Neoplastic / metabolism*
Cell Transformation, Neoplastic / pathology
Cross Reactions
ErbB Receptors / biosynthesis
ErbB Receptors / metabolism*
Forkhead Box Protein O1
Forkhead Transcription Factors
Humans
Mammary Neoplasms, Experimental / metabolism*
Mammary Neoplasms, Experimental / pathology
Mice
Molecular Sequence Data
Molecular Weight
Phosphoproteins / biosynthesis
Phosphoproteins / chemistry
Phosphoproteins / metabolism*
Receptor, ErbB-2 / biosynthesis
Receptor, ErbB-2 / metabolism*
Sequence Homology, Amino Acid
Transcription Factors / chemistry
Transcription Factors / immunology

Substances

Forkhead Box Protein O1
Forkhead Transcription Factors
Foxo1 protein, mouse
Phosphoproteins
STARD10 protein, human
Stard10 protein, mouse
Transcription Factors
ErbB Receptors
Receptor, ErbB-2