Format

Send to:

Choose Destination
See comment in PubMed Commons below
Appl Opt. 2004 May 10;43(14):2874-83.

Ultrahigh-resolution full-field optical coherence tomography.

Author information

  • 1Laboratoire d'Optique Physique, Ecole SupĂ©rieure de Physique et Chimie Industrielles, Centre National de la Recherche Scientifique, UnitĂ© Propre de Recherche A0005, 10 rue Vauquelin, F-75231 Paris Cedex 5, France. dubois@optique.espci.fr

Abstract

We have developed a white-light interference microscope for ultrahigh-resolution full-field optical coherence tomography of biological media. The experimental setup is based on a Linnik-type interferometer illuminated by a tungsten halogen lamp. En face tomographic images are calculated by a combination of interferometric images recorded by a high-speed CCD camera. Spatial resolution of 1.8 microm x 0.9 microm (transverse x axial) is achieved owing to the extremely short coherence length of the source, the compensation of dispersion mismatch in the interferometer arms, and the use of relatively high-numerical-aperture microscope objectives. A shot-noise-limited detection sensitivity of 90 dB is obtained in an acquisition time per image of 4 s. Subcellular-level images of plant, animal, and human tissues are presented.

PMID:
15143811
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Optical Society of America
    Loading ...
    Write to the Help Desk