Display Settings:


Send to:

Choose Destination
See comment in PubMed Commons below
FEBS Lett. 2004 May 7;565(1-3):101-5.

Identification of an Arabidopsis inorganic pyrophosphatase capable of being imported into chloroplasts.

Author information

  • 1Max-Planck-Institute of Molecular Plant Physiology, Golm, Germany.


An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl(2) was necessary for the protein's activity, whilst NaF inhibited it. The K(m) for pyrophosphate and the pH optimum of the protein was determined. The gene coding for this protein was expressed in all tissues, and its expression in rosette leaves was induced by incubation on metabolizable sugars. In vitro import experiments demonstrated that the protein could be imported into chloroplasts and localized to the stromal compartment.

[PubMed - indexed for MEDLINE]
Free full text
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk