Abstract
Unilateral microdialysis-perfusion of the preoptic area with 50 microM muscimol decreased the sleep period of rats to less than 3% of the baseline value over a 90 min period before death (p = 0.018 by Wilcoxon signed-rank test). These rats showed the expression of FOS in 36% of the orexin neurons located in the perifornical/lateral hypothalamic areas on the side ipsilateral to the perfusion site, but in only 3% of the orexin neurons on the side contralateral to it (p = 0.018 by Wilcoxon signed-rank test). These results suggest that subpopulations of the preoptic neurons give an inhibitory tone to the activities of the orexin neurons in the perifornical/lateral hypothalamic areas.
Copyright 2004 Lippincott Williams and Wilkins
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arousal / drug effects
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Arousal / physiology
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Carrier Proteins / analysis
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Carrier Proteins / biosynthesis*
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Gene Expression Regulation / physiology
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Injections, Intraventricular
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Intracellular Signaling Peptides and Proteins*
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Male
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Muscimol / administration & dosage*
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Neurons / chemistry
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Neurons / drug effects*
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Neurons / metabolism
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Neuropeptides / analysis
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Neuropeptides / biosynthesis*
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Orexins
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Perfusion / methods
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Preoptic Area / chemistry
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Preoptic Area / drug effects*
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Preoptic Area / metabolism
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Proto-Oncogene Proteins c-fos / analysis
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Proto-Oncogene Proteins c-fos / biosynthesis*
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Rats
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Rats, Sprague-Dawley
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Sleep / drug effects
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Sleep / physiology
Substances
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Carrier Proteins
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Intracellular Signaling Peptides and Proteins
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Neuropeptides
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Orexins
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Proto-Oncogene Proteins c-fos
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Muscimol