FOS expression in orexin neurons following muscimol perfusion of preoptic area

Neuroreport. 2004 May 19;15(7):1127-31. doi: 10.1097/00001756-200405190-00009.

Abstract

Unilateral microdialysis-perfusion of the preoptic area with 50 microM muscimol decreased the sleep period of rats to less than 3% of the baseline value over a 90 min period before death (p = 0.018 by Wilcoxon signed-rank test). These rats showed the expression of FOS in 36% of the orexin neurons located in the perifornical/lateral hypothalamic areas on the side ipsilateral to the perfusion site, but in only 3% of the orexin neurons on the side contralateral to it (p = 0.018 by Wilcoxon signed-rank test). These results suggest that subpopulations of the preoptic neurons give an inhibitory tone to the activities of the orexin neurons in the perifornical/lateral hypothalamic areas.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Arousal / drug effects
  • Arousal / physiology
  • Carrier Proteins / analysis
  • Carrier Proteins / biosynthesis*
  • Gene Expression Regulation / physiology
  • Injections, Intraventricular
  • Intracellular Signaling Peptides and Proteins*
  • Male
  • Muscimol / administration & dosage*
  • Neurons / chemistry
  • Neurons / drug effects*
  • Neurons / metabolism
  • Neuropeptides / analysis
  • Neuropeptides / biosynthesis*
  • Orexins
  • Perfusion / methods
  • Preoptic Area / chemistry
  • Preoptic Area / drug effects*
  • Preoptic Area / metabolism
  • Proto-Oncogene Proteins c-fos / analysis
  • Proto-Oncogene Proteins c-fos / biosynthesis*
  • Rats
  • Rats, Sprague-Dawley
  • Sleep / drug effects
  • Sleep / physiology

Substances

  • Carrier Proteins
  • Intracellular Signaling Peptides and Proteins
  • Neuropeptides
  • Orexins
  • Proto-Oncogene Proteins c-fos
  • Muscimol