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    J Immunol. 2004 May 15;172(10):6382-7.

    Inactivation of C5a anaphylatoxin by a peptide that is complementary to a region of C5a.

    Fujita E, Farkas I, Campbell W, Baranyi L, Okada H, Okada N.

    Source

    Department of Biodefense, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.

    Abstract

    PL37 (RAARISLGPRCIKAFTE) is an antisense homology box peptide composed of aa 37-53 of C5a-anaphylatoxin and is considered to be the region essential for C5a function. Using a computer program, we designed the complementary peptides ASGAPAPGPAGPLRPMF (Pep-A) and ASTAPARAGLPRLPKFF (Pep-B). Pep-A bound to PL37 and to C5a with very slow dissociation as determined by analysis using surface plasmon resonance, whereas Pep-B failed to bind at all. C5a was inactivated by concentrations of 7 nM or more of Pep-A, and this concentration of Pep-A inhibited induction of intracellular Ca(2+) influx in neutrophils. Patch clamp electrophysiology experiments also showed the effectiveness of Pep-A in C5aR-expressing neuroblastoma cells. Furthermore, Pep-A administration prevented rats from C5a-mediated rapid lethal shock induced by an Ab to a membrane inhibitor of complement after LPS sensitization.

    PMID:
    15128829
    [PubMed - indexed for MEDLINE]
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