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Phys Med Biol. 2004 Apr 7;49(7):1227-34.

Three-dimensional cellular-level imaging using full-field optical coherence tomography.

Author information

  • 1Laboratoire d'Optique Physique, Ecole SupĂ©rieure de Physique et Chimie Industrielles, Centre National de la Recherche Scientifique, UPR A0005, 10 rue Vauquelin, F-75231 Paris Cedex 5, France.

Abstract

An ultrahigh-resolution full-field optical coherence tomography (OCT) system has been developed for cellular-level imaging of biological media. The system is based on a Linnik interference microscope illuminated with a tungsten halogen lamp, associated with a high-resolution CCD camera. En face tomographic images are produced in real time, with the best spatial resolution ever achieved in OCT (0.7 microm x 0.9 microm, axial x transverse). A shot-noise limited detection sensitivity of 80 dB can be reached with an acquisition time per image of 1 s. Images of animal ophthalmic biopsies and vegetal tissues are shown.

PMID:
15128200
[PubMed - indexed for MEDLINE]
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