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Genome Res. 2004 May;14(5):878-85.

A versatile assay for high-throughput gene expression profiling on universal array matrices.

Author information

  • 1Illumina, Inc., San Diego, California 92121, USA. jfan@illumina.com

Abstract

We report a flexible, sensitive, and quantitative gene-expression profiling system for assaying more than 400 genes, with three probes per gene, for 96 samples in parallel. The cDNA-mediated annealing, selection, extension and ligation (DASL) assay targets specific transcripts, using oligonucleotides containing unique address sequences that can hybridize to universal arrays. Cell-specific gene expression profiles were obtained using this assay for hormone-treated cell lines and laser-capture microdissected cancer tissues. Gene expression profiles derived from this assay were consistent with those determined by qRT-PCR. The DASL assay has been automated for use with a bead-based 96-array matrix system. The combined high-throughput assay and readout system is accurate and efficient, and can cost-effectively profile the expression of hundreds of genes in thousands of samples.

PMID:
15123585
[PubMed - indexed for MEDLINE]
PMCID:
PMC479115
Free PMC Article
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