Plasma intestinal fatty acid binding protein (I-FABP) concentrations increase following intestinal ischemia in pigs

Res Vet Sci. 2004 Aug;77(1):89-91. doi: 10.1016/j.rvsc.2004.02.006.

Abstract

Intestinal fatty acid binding protein (I-FABP) is an intracellular epithelial protein in the intestinal mucosa of many animals. I-FABP appears in the circulation following epithelial damage, and in humans, is proven to be a parameter for damage to the mucosa. In this paper, an ELISA test designed for human I-FABP analysis was used to assay pig blood samples. The test recognized I-FABP cloned from pig small intestine and expressed in Escherichia coli. Furthermore, in our experimental model of (low flow) intestinal ischemia and reperfusion a significant rise in plasma I-FABP concentrations 15-30 min after clamping of the mesenteric artery was demonstrated. This is the first report that in pigs circulating I-FABP is a useful marker for (mild) intestinal injury, and could possibly be used to monitor (intestinal) health in clinical practice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins / blood*
  • Enzyme-Linked Immunosorbent Assay / veterinary
  • Fatty Acid-Binding Proteins
  • Intestinal Mucosa / blood supply*
  • Male
  • Mesenteric Artery, Superior
  • Reperfusion Injury / blood
  • Reperfusion Injury / veterinary*
  • Swine
  • Swine Diseases / blood*

Substances

  • Carrier Proteins
  • Fatty Acid-Binding Proteins