Antibacterial and antifungal activities of acetone and dimethyl sulfoxide (DMSO) extracts of 45 different propolis samples from the Mugla province of Turkey were investigated. Antimicrobial activity of propolis varied depending on propolis sample, dosage of propolis, and the extraction solvents for all test microorganisms. Antimicrobial activity of all propolis samples increased with increasing dosage without reaching a plateau at the highest dosage tested. Except for Brucella melitensis, the DMSO extracts of all propolis samples were more active than the acetone extracts of the same samples. For B. melitensis, the acetone extracts of all propolis samples showed greater activity. The most sensitive microorganism to propolis was Shigella sonnei in the gram-negative group and Streptococcus mutans in the gram-positive group. The least sensitive microorganism was Candida albicans. A control test run with standard antibiotics revealed that propolis samples from the Mugla province of Turkey has a similar or greater inhibitory effect on S. mutans, Salmonella typhi, Pseudomonas aeruginosa, S. sonnei, and C. albicans growth.