Reversible immobilization of engineered molecules by Ni-NTA chelators

Bioelectrochemistry. 2004 Jun;63(1-2):271-5. doi: 10.1016/j.bioelechem.2003.10.024.

Abstract

Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)(6)-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor micro-array.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Biosensing Techniques / methods*
  • Chelating Agents / chemistry
  • Coated Materials, Biocompatible / chemistry*
  • Electrochemistry / methods
  • Electrodes
  • Light
  • Nitrilotriacetic Acid / analogs & derivatives*
  • Nitrilotriacetic Acid / chemistry*
  • Organometallic Compounds / chemistry*
  • Photosystem II Protein Complex / analysis*
  • Photosystem II Protein Complex / chemistry*
  • Photosystem II Protein Complex / genetics
  • Photosystem II Protein Complex / radiation effects
  • Protein Engineering / methods*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / radiation effects

Substances

  • Chelating Agents
  • Coated Materials, Biocompatible
  • Organometallic Compounds
  • Photosystem II Protein Complex
  • Recombinant Proteins
  • nickel nitrilotriacetic acid
  • Nitrilotriacetic Acid