Abstract
Electrochemical synthesis of nickel-nitrilotriacetic acid (Ni-NTA) chelators, for subsequent immobilization of (His)(6)-tagged proteins (Photosystem II (PSII) as model molecule), on Au or Au-graphite electrodes is compared to chemical synthesis. Results show: (i) higher Ni-NTA surface density, (ii) shorter treatment time (1-12 min vs. 16 h normally needed for self-assembled monolayer (SAM)), (iii) possibility of addressing the chelator to only one Au electrode, in a sensor micro-array.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adsorption
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Biosensing Techniques / methods*
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Chelating Agents / chemistry
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Coated Materials, Biocompatible / chemistry*
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Electrochemistry / methods
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Electrodes
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Light
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Nitrilotriacetic Acid / analogs & derivatives*
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Nitrilotriacetic Acid / chemistry*
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Organometallic Compounds / chemistry*
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Photosystem II Protein Complex / analysis*
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Photosystem II Protein Complex / chemistry*
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Photosystem II Protein Complex / genetics
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Photosystem II Protein Complex / radiation effects
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Protein Engineering / methods*
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Recombinant Proteins / chemistry
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Recombinant Proteins / radiation effects
Substances
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Chelating Agents
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Coated Materials, Biocompatible
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Organometallic Compounds
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Photosystem II Protein Complex
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Recombinant Proteins
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nickel nitrilotriacetic acid
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Nitrilotriacetic Acid