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Nucleic Acids Res. 2004 Apr 16;32(7):2123-8. Print 2004.

The RNA-dependent RNA polymerase, QDE-1, is a rate-limiting factor in post-transcriptional gene silencing in Neurospora crassa.

Forrest EC, Cogoni C, Macino G.

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Istituto Pasteur e Fondazione Cenci Bolognetti, Dipartimento di Biotecnologie Cellulari ed Ematologia, Sezione di Genetica Molecolare, Universitá di Roma La Sapienza, 00161 Roma, Italy.

Abstract

The RNA-dependent RNA polymerase (RdRP) qde-1 is an essential component of post-transcriptional gene silencing (PTGS), termed 'quelling' in the fungus Neurospora crassa. Here we show that the overexpression of QDE-1 results in a dramatic increase in the efficiency of quelling, with a concomitant net increase in the quantity of al-1 siRNAs. Moreover, in overexpressed strains there is a significant reduction in the number of transgenes required to induce quelling, and an increase in the phenotypic stability despite progressive loss of tandemly repeated transgenes, which normally determines reversion of a silenced phenotype to wild type. These data suggest that the activation and maintenance of silencing in Neurospora appear to rely both on the cellular amount of QDE-1 and the amount of transgenic copies producing RNA molecules that act as a substrate for the RdRP, implicating QDE-1 as a rate-limiting factor in PTGS.

PMID:: 15090622; [PubMed - indexed for MEDLINE]
PMCID:: PMC407816

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Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase. [Nature. 1999]
Gene silencing in Neurospora crassa requires a protein homologous to RNA-dependent RNA polymerase.
Cogoni C, Macino G. Nature. 1999 May 13; 399(6732):166-9.
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Review Quelling in Neurospora crassa.
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Review Quelling: post-transcriptional gene silencing guided by small RNAs in Neurospora crassa.
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Cited by 8 PubMed Central articles

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