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Curr Opin Biotechnol. 2004 Apr;15(2):132-8.

A tale of two integrations, transgene and T-DNA: gene targeting by homologous recombination in rice.

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  • 1National Institute for Basic Biology, Okazaki 444-8585, Japan.


The first successful and reproducible gene targeting by homologous recombination, without the concomitant occurrence of ectopic events, has been reported. This will be a powerful approach for the characterization of gene function in rice, an important crop and a model for other cereal species. Models have been proposed to explain gene replacement by homologous recombination, including a possible model for Agrobacterium-mediated gene targeting using a strong positive-negative selection.

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