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Anal Chem. 2004 Apr 15;76(8):2406-10.

Quantum dot-encoded mesoporous beads with high brightness and uniformity: rapid readout using flow cytometry.

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  • 1Wallace H. Coulter Department of Biomedical Engineering, Emory University and Georgia Institute of Technology, 1639 Pierce Drive, Suite 2001, Atlanta, Georgia 30322, USA.


A new generation of optically encoded beads has been prepared by using mesoporous polystyrene beads and surfactant-coated semiconductor quantum dots. In comparison with nonporous beads of similar sizes and chemical compositions, the encoded porous beads are approximately 1000 times brighter and 5 times more uniform in fluorescence intensities. Using both absolute intensity and ratiometric fluorescence coding, we show that the beads can be identified with a standard flow cytometer at 1000 beads/s. This result indicates that the multiple excited-state lifetimes and relaxation pathways of quantum dots do not limit their applications in high-speed optical detection and imaging.

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