Bioinformatics. 2004 Oct 12;20(15):2473-5. Epub 2004 Apr 8.

G-PRIMER: greedy algorithm for selecting minimal primer set.

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BioInformatics Institute, 30 Biopolis Street, #07-01 Matrix, Singapore 138671. jiren@bii.a-star.edu.sg

Abstract

G-PRIMER, a web-based primer design program, has been developed to compute a minimal primer set specifically annealed to all the open reading frames in a given microbial genome. This program has been successfully used in the microarray experiment for analyzing the expression of genes in the Xanthomonas campestris genome. AVAILABILITY: It is available at http://mammoth.bii.a-star.edu.sg/gprimer/. Its source code is available upon request.

PMID:: 15073009; [PubMed - indexed for MEDLINE]

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Cited by 3 PubMed Central articles

Confronting two-pair primer design for enzyme-free SNP genotyping based on a genetic algorithm. [BMC Bioinformatics. 2010]
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