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Bioinformatics. 2004 Oct 12;20(15):2471-2. Epub 2004 Apr 8.

PerlPrimer: cross-platform, graphical primer design for standard, bisulphite and real-time PCR.

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  • 1Murdoch Childrens Research Institute, Royal Childrens Hospital, Flemington Road, Parkville, Vic., 3052, Australia.


PerlPrimer is a cross-platform graphical user interface application for the design of primers for standard, bisulphite and real-time PCR, and sequencing. The program incorporates highly accurate melting-temperature and primer-dimer prediction algorithms with powerful tools such as sequence retrieval from Ensembl and the ability to BLAST search primer pairs. It aims to automate and simplify the process of primer design.


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