We evaluated the new automated glycohemoglobin analyzer HLC-723G7 (G7) intended for beta-thalassemia diagnosis to determine correlation of hemoglobin (Hb) F and Hb A2 quantitation with the Variant beta-Thalassemia Short Program and to evaluate the performance of the G7 analyzer. Two hundred fifty EDTA blood samples with Hb A, A2, and F by Variant were analyzed for Hb A2 and Hb F by G7 device. Recovery, precision, and interference of the G7 analyzer were studied. The R2 values for Hb A2 and Hb F were 0.963 and 0.640, respectively. Recovery of Hb A2 and Hb F of low and high control samples ranged from 96% to 100% and 91% to 101%, respectively. The coefficients of variation (CVs) of intraassay precision ranged from 1.3% to 3.9% for Hb F and 0.0 to 1.9% for Hb A2. The CVs of interassay precision ranged from 1.9% to 5.6% for Hb F and 2.5% to 3.0% for Hb A2. The CVs for Hb F in both conjugated bilirubin and chyle substance groups were higher than the CVs for Hb A2. This evaluation revealed very good correlation of Hb A2 measurement between the G7 and the Variant devices. The performance evaluation demonstrated good recovery, precision, and low interference of both Hb A2 and Hb F measurements.