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Curr Opin Chem Biol. 2004 Feb;8(1):66-75.

Chemical probes and tandem mass spectrometry: a strategy for the quantitative analysis of proteomes and subproteomes.

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  • 1Institute for Systems Biology, 1441 N 34th Street, Seattle, Washington 98103-8904, USA.

Abstract

Quantitative proteome profiling using mass spectrometry and stable isotope dilution is being widely applied for the functional analysis of biological systems and for the detection of clinical, diagnostic or prognostic marker proteins. Because of the enormous complexity of proteomes, their comprehensive analysis is unlikely to be routinely achieved in the near future. However, in recent years, significant progress has been achieved focusing quantitative proteomic analyses on specific protein classes or subproteomes that are rich in biologically or clinically important information. Such projects typically combine the use of chemical probes that are specific for a targeted group of proteins and may contain stable isotope signatures for accurate quantification with automated tandem mass spectrometry and bioinformatics tools for data analysis. In this review, we summarize technical and conceptual advances in quantitative subproteome profiling based on tandem mass spectrometry and chemical probes.

PMID:
15036159
[PubMed - indexed for MEDLINE]
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