Ann N Y Acad Sci. 2003 Dec;1010:557-9.

The role of apoptosis in acetaminophen-induced injury.

Kass GE, Macanas-Pirard P, Lee PC, Hinton RH.

Source

School of Biomedical and Life Sciences, University of Surrey, Guildford, Surrey GU2 7XH, United Kingdom. g.kass@surrey.ac.uk

Abstract

Apoptosis plays a critical role in acetaminophen (AAP)-induced hepatic injury, since inhibiting apoptosis also prevents the development of acute liver failure. In this study, the mechanism of apoptosis induction by AAP was investigated in the human hepatoblastoma cell line HuH7. AAP caused marked cytotoxicity in HuH7 cells as a result of apoptosis. Processing of execution caspases to their corresponding active fragments and cleavage of cytokeratin-18 were observed, supporting a role of caspases in AAP-induced apoptosis. The manifestation of apoptosis was preceded by a translocation of cytochrome c from mitochondria to the cytosol. In conclusion, AAP induces apoptosis in human hepatoblastoma HuH7 cells through mitochondrial cytochrome c release and caspase activation.

PMID:: 15033790; [PubMed - indexed for MEDLINE]

MeSH Terms, Substances

MeSH Terms

Substances

Acetaminophen

LinkOut - more resources

Full Text Sources

Other Literature Sources

Molecular Biology Databases

ACETAMINOPHEN - HSDB

Supplemental Content

Related citations

Glycogen synthase kinase-3 mediates acetaminophen-induced apoptosis in human hepatoma cells. [J Pharmacol Exp Ther. 2005]
Glycogen synthase kinase-3 mediates acetaminophen-induced apoptosis in human hepatoma cells.
Macanas-Pirard P, Yaacob NS, Lee PC, Holder JC, Hinton RH, Kass GE. J Pharmacol Exp Ther. 2005 May; 313(2):780-9. Epub 2005 Jan 21.
Involvement of mitochondria in acetaminophen-induced apoptosis and hepatic injury: roles of cytochrome c, Bax, Bid, and caspases. [Toxicol Appl Pharmacol. 2003]
Involvement of mitochondria in acetaminophen-induced apoptosis and hepatic injury: roles of cytochrome c, Bax, Bid, and caspases.
El-Hassan H, Anwar K, Macanas-Pirard P, Crabtree M, Chow SC, Johnson VL, Lee PC, Hinton RH, Price SC, Kass GE. Toxicol Appl Pharmacol. 2003 Sep 1; 191(2):118-29.
Chemoprotective effect of insulin-like growth factor I against acetaminophen-induced cell death in Chang liver cells via ERK1/2 activation. [Toxicology. 2007]
Chemoprotective effect of insulin-like growth factor I against acetaminophen-induced cell death in Chang liver cells via ERK1/2 activation.
Hwang HJ, Kwon MJ, Nam TJ. Toxicology. 2007 Jan 25; 230(1):76-82. Epub 2006 Dec 12.
Review The role of oxidant stress and reactive nitrogen species in acetaminophen hepatotoxicity. [Toxicol Lett. 2003]
Review The role of oxidant stress and reactive nitrogen species in acetaminophen hepatotoxicity.
Jaeschke H, Knight TR, Bajt ML. Toxicol Lett. 2003 Oct 15; 144(3):279-88.
Review Apoptosis and necrosis in the liver: a tale of two deaths? [Hepatology. 2006]
Review Apoptosis and necrosis in the liver: a tale of two deaths?
Malhi H, Gores GJ, Lemasters JJ. Hepatology. 2006 Feb; 43(2 Suppl 1):S31-44.

See reviews... See all...

Related information

Related Citations
Calculated set of PubMed citations closely related to the selected article(s) retrieved using a word weight algorithm. Related articles are displayed in ranked order from most to least relevant, with the “linked from” citation displayed first.
Compound (MeSH Keyword)
PubChem chemical compound records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.
Substance (MeSH Keyword)
PubChem chemical substance (submitted) records that are classified under the same Medical Subject Headings (MeSH) controlled vocabulary as the current articles.

Recent activity

Clear Turn Off Turn On

The role of apoptosis in acetaminophen-induced injury.
The role of apoptosis in acetaminophen-induced injury.
Ann N Y Acad Sci. 2003 Dec ;1010:557-9.

PubMed
Guidelines on the use of intravenous immune globulin for neurologic conditions.
Guidelines on the use of intravenous immune globulin for neurologic conditions.
Transfus Med Rev. 2007 Apr ;21(2 Suppl 1):S57-107.

PubMed
Web-based therapist-assisted cognitive behavioral treatment of panic symptoms: a...
Web-based therapist-assisted cognitive behavioral treatment of panic symptoms: a randomized controlled trial with a three-year follow-up.
J Anxiety Disord. 2010 May ;24(4):387-96. Epub 2010 Feb 17 .

PubMed
Resolving cell population heterogeneity: real-time PCR for simultaneous multiple...
Resolving cell population heterogeneity: real-time PCR for simultaneous multiplexed gene detection in multiple single-cell samples.
PLoS One. 2009 Jul 27 ;4(7):e6326.

PubMed
Epidural ropivacaine concentrations for intraoperative analgesia during major up...
Epidural ropivacaine concentrations for intraoperative analgesia during major upper abdominal surgery: a prospective, randomized, double-blinded, placebo-controlled study.
Anesth Analg. 2009 Jun ;108(6):1971-6.

PubMed

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

PubMed

Display Settings:

Send to: