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Eur J Pharmacol. 2004 Mar 8;487(1-3):205-11.

Muscarinic receptors mediating contraction of female mouse urinary bladder: effects of oestrogen.

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  • 1Department of Pharmacology, Monash University, Clayton 3800, Victoria, Australia.


Muscarinic receptors mediating contraction of bladder detrusor muscle from female mice were examined. Mice were untreated (A) or treated with oestradiol cypionate (200 microg/kg) 24 h (B) or 96 h (C) before experimentation, or were pregnant (day 17) (D). Saturation radioligand binding experiments using [(3)H]quinuclidinyl benzilate ([(3)H] QNB) indicated similar muscarinic receptor densities and affinities in bladders from groups A and B. Neither oestrogen treatment nor pregnancy altered pD(2) estimates for methacholine. Maximum responses to methacholine and high-K(+) physiological salt solution (KPSS) were significantly greater (P<0.05) in tissues from groups C and D than in A and B. Potencies of other muscarinic receptor agonists were similar in groups A and B with an order of acetylcholine plus physostigmine (10 microM) approximately methacholine plus physostigmine (10 microM)>methacholine approximately acetylcholine>bethanechol. Antagonist pK(B) estimates were similar in bladders from groups A and B with a rank order of: atropine>/=4-diphenyl acetoxy-N-methyl piperidine methiodide>parafluorohexahydrosiladifenidol approximately pirenzepine>himbacine, implicating muscarinic M(1) and/or M(5) as well as muscarinic M(3) receptors in mediating methacholine-induced bladder contraction.

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