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Emerg Infect Dis. 2004 Feb;10(2):311-6.

Real-time reverse transcription-polymerase chain reaction assay for SARS-associated coronavirus.

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  • 1Centers for Disease Control and Prevention, Atlanta, Georgia, USA.

Abstract

A real-time reverse transcription-polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome-associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.

PMID:
15030703
[PubMed - indexed for MEDLINE]
PMCID:
PMC3322901
Free PMC Article
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