A polyherbal ayurvedic formulation from an ancient authentic classical text of ayurveda was evaluated for its activity against inflammatory bowel disease (IBD). The polyherbal formulation contained four different drugs viz., Bilwa (Aegle marmeloes), Dhanyak (Coriandrum sativum), Musta (Cyperus rotundus) and Vala (Vetiveria zinzanioids). The formulation has been tried before in clinical practice and was found to be useful in certain number of cases of IBD (ulcerative colitis), so was tried in the same form i.e., decoction (aqueous extract) in experimental animals to revalidate the claims of the same. The formulation was tried on two different experimental animal models of inflammatory bowel disease, which are acetic acid-induced colitis in mice and indomethacin-induced enterocolitis in rats. Prednisolone was used as the standard drug for comparison. The formulation showed significant inhibitory activity against inflammatory bowel disease induced in these experimental animal models. The activity was comparable with the standard drug prednisolone. The results obtained established the efficacy of this polyherbal formulation against inflammatory bowel diseases.