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Zhonghua Wai Ke Za Zhi. 2004 Jan 22;42(2):104-6.

[HBx gene facilitates the proliferation activity of hepatoma cells in vitro and in vivo].

[Article in Chinese]

Author information

  • 1Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

Abstract

OBJECTIVE:

To investigate the effects of HBx gene on proliferation activity of hepatoma cells in vitro and in vivo.

METHODS:

The plasmid pHA-HBx carrying HBx gene was transfected into HepG(2) cells, and the positive clones were screened and identified with G418 and RT-PCR, respectively. The growth curve and population doubling time were calculated, and the cell cycle was analyzed by flow cytometry (FCM). The proliferation activity of transformed cells was measured with (3)H-TdR incorporation rate and nude mice model in vitro and in vivo.

RESULTS:

The result of RT-PCR indicated that HBx gene was integrated into the genome DNA of HepG(2) cells and transcripted. The growth curve and population doubling time showed a high proliferation activity of transformed cells. The amount of cells at stage S and G(2)/M were significantly higher, and cells at stage G(0)/G(1) were lower than those in control group. The tumors developed from transfected cells grew much quicker than those developed from HepG(2) cells in nude mice model.

CONCLUSION:

HBx gene can facilitate the proliferation of hepatoma cells both in vitro and in vivo.

PMID:
15009993
[PubMed - indexed for MEDLINE]
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