J Infect Dis. 1992 Sep;166(3):620-2.

Concordance of human immunodeficiency virus detection by polymerase chain reaction and by serologic assays in a Dutch cohort of seronegative homosexual men.

Bruisten SM, Koppelman MH, Dekker JT, Bakker M, de Goede RE, Roos MT, de Wolf F, Coutinho RA, Goudsmit J, Huisman HG.

Source

Central Laboratory of the Netherlands Red Cross Blood Transfusion Service, Amsterdam.

Abstract

In three subgroups of a clinically and socially well defined group of Dutch homosexual men, the prevalence of human immunodeficiency virus type 1 (HIV-1) sequences in seronegative blood samples was studied using the polymerase chain reaction (PCR). In 19 seronegative partners of seropositive persons, no HIV-1 sequences were found by PCR in either early (1984/1985) or more recent (1987) samples. In 42 seronegative persons selected by their high risk for HIV-1 infection, none harbored HIV-1 sequences in either early (1985/1986) or late (1989) samples. In 15 people who seroconverted for HIV-1, only 2 samples collected 3 months before seroconversion were PCR-positive. These persons were also HIV antigen-positive at this time. These data suggest that a latent infection greater than 6 months does not occur and that the combination of HIV antibody and HIV antigen tests is appropriate and conclusive in most cases of HIV-1 infection.

PMID:: 1500745; [PubMed - indexed for MEDLINE]

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Publication Types

Research Support, Non-U.S. Gov't

MeSH Terms

Substances

DNA, Viral

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