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Appl Environ Microbiol. 2004 Mar;70(3):1869-73.

L-alanine auxotrophy of Lactobacillus johnsonii as demonstrated by physiological, genomic, and gene complementation approaches.

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  • 1NestlĂ© Research Centre, Nestec, Ltd., CH-1000 Lausanne 26, Switzerland.

Abstract

Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by heterologous expression of the Bacillus subtilis L-alanine dehydrogenase.

PMID:
15006820
[PubMed - indexed for MEDLINE]
PMCID:
PMC368417
Free PMC Article

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