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Chemosphere. 2004 Apr;55(3):395-400.

Evaluation of chloroform/methanol and dichloromethane/hexane extractable lipids as surrogate measures of sample partition capacity for organochlorines in fish tissues.

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  • 1Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Avenue, Windsor, ON, Canada N9B 3P4. kgd@uwindsor.ca

Abstract

Organic contaminant concentration data are often lipid normalized to provide an indication of the chemical fugacity in the sample or to compare the relative equilibrium status of samples from the same environment. This study compared lipid normalized PCB and organochlorine concentration data for tissues of individual fish when lipids were measured using chloroform/methanol (CM) or dichloromethane/hexane (DH) extractions. The CM extraction produced higher lipid yields for dorsal muscle (8-fold difference) and carcass (1.7-fold difference) compared to DH extractions, while both methods yielded comparable lipid contents for liver and adipose tissue. Lipid normalized tissue/carcass PCB and organochlorine concentration ratios were variable across tissue types when lipid contents from the CM technique were used, whereas, tissue/carcass concentration ratios approached the expected value of unity for each tissue when DH-derived lipids were used. These data suggest that the tissue lipid content as derived using the DH extraction provided a better surrogate measure of sample partition capacity than tissue lipids determined using the CM technique.

PMID:
14987938
[PubMed - indexed for MEDLINE]
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