Format

Send to:

Choose Destination
See comment in PubMed Commons below
Methods Mol Biol. 2004;263:33-44.

Multiparameter flow cytometry of bacteria.

Author information

  • 1The Center for Microbial Cytometry and Howard M. Shapiro, MD, PC, West Newton, NY, USA.

Abstract

The small size of bacteria makes some microbial constituents undetectable or measurable with only limited precision by flow cytometry. Bacteria may also behave differently from eukaryotes in terms of their interaction with dyes, drugs, and other reagents. It is therefore difficult to design multiparameter staining protocols that work, unmodified, across a wide range of bacterial species. This chapter describes reliable flow cytometric methods for assessment of the physiologic states of Gram-negative organisms, on the one hand, and Gram-positive organisms, on the other, based on measurement of membrane potential and membrane permeability. These techniques are useful in the assessment of effects of environmental conditions and antimicrobial agents on microorganisms.

PMID:
14976359
[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Icon for Springer
    Loading ...
    Write to the Help Desk