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Mol Cell. 2004 Feb 13;13(3):389-401.

Association of Mre11p with double-strand break sites during yeast meiosis.

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  • 1Génétique Moléculaire de la Recombinaison, Institut Curie, Section de Recherche, UMR144 CNRS, 26 rue d'Ulm, 75248 Paris Cedex 05, France.

Abstract

The repair of DNA double-strand breaks (DSBs) requires the activity of the Mre11/Rad50/Xrs2(Nbs1) complex. In Saccharomyces cerevisiae, this complex is required for both the initiation of meiotic recombination by Spo11p-catalyzed programmed DSBs and for break end resection, which is necessary for repair by homologous recombination. We report that Mre11p transiently associates with the chromatin of Spo11-dependent DSB regions throughout the genome. Mutant analyses show that Mre11p binding requires the function of all genes required for DSB formation, with the exception of RAD50. However, Mre11p binding does not require DSB formation itself, since Mre11p transiently associates with DSB regions in the catalysis-negative mutant spo11-Y135F. Mre11p release from chromatin is blocked in mutants that accumulate unresected DSBs. We propose that Mre11p is a component of a pre-DSB complex that assembles on the DSB sites, thus ensuring a tight coupling between DSB formation by Spo11p and the processing of break ends.

PMID:
14967146
[PubMed - indexed for MEDLINE]
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