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Bioinformatics. 2004 Jul 10;20(10):1644-5. Epub 2004 Feb 12.

Amplicon: software for designing PCR primers on aligned DNA sequences.

Author information

  • Australian Antarctic Division, Channel Highway, Kingston, TAS, 7050, Australia. simon.jarman@aad.gov.au

Abstract

SUMMARY:

Amplicon is a program for designing PCR primers on aligned groups of DNA sequences. The most important application for Amplicon is the design of 'group-specific' PCR primer sets that amplify a DNA region from a given taxonomic group but do not amplify orthologous regions from other taxonomic groups.

AVAILABILITY:

Amplicon is freely available as a script that will run on any platform with Python 2.3 installed (http://www.python.org). It is also available as a Windows executable. Free downloads that do not require registration can be found at http://www.aad.gov.au/amplicon

PMID:
14962918
[PubMed - indexed for MEDLINE]
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