Methods. 2004 Mar;32(3):265-70.

Assays for measuring in vitro basophil activation induced by recombinant allergens.

Valent P, Hauswirth AW, Natter S, Sperr WR, Bühring HJ, Valenta R.

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Division of Hematology and Hemostaseology, Department of Internal Medicine I, The University of Vienna, Vienna, Austria. peter.valent@akh-wien.ac.at

Abstract

The diagnosis of type I allergy is essentially based on clinical data, skin tests, and measurements of allergen-specific IgE. However, the determination of specific IgE per se does not permit a definitive conclusion concerning the response of effector cells to the respective allergen(s) and consecutive clinical symptoms in all patients. In an attempt to overcome this problem, a number of basophil-activation assays have been developed during the last few years. Today, allergen-induced activation of blood basophils can be employed as a specific and reliable measure of IgE-dependent responses in sensitized individuals. Using recombinant allergens and basophil-specific markers, these novel assays appear to serve as simple and useful tests in component-resolved diagnosis of type I allergies. In the current article, the biochemical, functional, and technical background of these basophil tests is discussed.

PMID:: 14962761; [PubMed - indexed for MEDLINE]

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