A simple, reliable, and fast protocol for thraustochytrid DNA extraction

C Mo; B Rinkevich

doi:10.1007/s101260000069

A simple, reliable, and fast protocol for thraustochytrid DNA extraction

Mar Biotechnol (NY). 2001 Mar;3(2):100-2. doi: 10.1007/s101260000069.

Authors

C Mo¹, B Rinkevich

Affiliation

¹ Minerva Center for Marine Invertebrate Immunology and Developmental Biology, Israel Oceanographic and Limnological Research, National Institute of Oceanography, P.O. Box 8030, Tel Shikmona, Haifa 31080, Israel.

PMID: 14961371
DOI: 10.1007/s101260000069

Abstract

DNA extraction of thraustochytrids, common marine unicellular organisms, is usually accomplished by either the cetyltrimethylammonium bromide (CTAB) or proteinase K protocols. A novel lysis buffer protocol for thraustochytrid total DNA extraction is described. The average isolated total DNA is 20 to 40 kb, and DNA samples are suitable for a variety of uses including 18S-ribosomal DNA polymerase chain reaction, restriction enzyme digestions, and amplified fragment length polymorphism analyses. The new protocol is also faster than the other protocols.