Relative base excision repair in Xiphophorus fish tissue extracts

R B Walter; H M Sung; R D Obermoeller; D L Mitchell; G W Intano; C A Walter

doi:10.1007/s10126-001-0027-0

Relative base excision repair in Xiphophorus fish tissue extracts

Mar Biotechnol (NY). 2001 Jun;3(Supplement 1):S50-60. doi: 10.1007/s10126-001-0027-0.

Authors

R B Walter¹, H M Sung, R D Obermoeller, D L Mitchell, G W Intano, C A Walter

Affiliation

¹ Department of Chemistry and Biochemistry, Southwest Texas State University, 419 Centennial Hall, 601 University Drive, San Marcos, TX 78666-4616, USA. RWALTER@swt.edu

PMID: 14961300
DOI: 10.1007/s10126-001-0027-0

Abstract

To begin characterizing DNA repair capability among Xiphophorus species, we adapted oligonucleotide-based DNA repair assays to extracts of fish tissues. Here, we report the initial results of relative base excision repair (BER) capability among 3 inbred Xiphophorus fish lines representing 2 species (X. maculatus and X. couchianus), and interspecies hybrid F(1) animals produced by crossing them. Overall, data from uracil- N-glycosylase (UNG)-initiated BER assay (UNG-BER) indicate that brain tissue extracts generally possess higher BER activity than do gill and liver extracts. UNG-BER activities in gill and liver extracts were similar. The BER activity in the tissues of F(1) interspecies hybrids followed the activity pattern of the X. couchianus parent in gill and liver extracts, was similar to the X. maculatus parent in brain extracts, but exhibited a reduced repair capacity in gill tissue extracts compared with either parent. We discuss the use of oligonucleotide-based DNA repair assays to elucidate the role that inheritance of DNA repair potential may play in susceptibility to disease and tumorigenesis in the intact organism.