Abstract
The use of recombinant antigens is essential for the construction of robust and sensitive diagnostic assays. A critical step in the preparation of recombinant antigens is protein purification. Purification problems may be very different for related structural proteins expressed in the same host or for the same protein expressed in different hosts, because the biochemical characteristics of a recombinant protein, expressed in a heterologous system, are unique. In this chapter we make a brief introduction to protein purification procedures and we present a quick purification process suitable for the isolation of recombinant protein having high isoelectric points encoding non-conformational epitopes.
MeSH terms
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Antibodies, Viral / blood
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Antigens / chemistry
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Antigens / genetics
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Antigens / isolation & purification
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Chemical Precipitation
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Chromatography, Ion Exchange
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Escherichia coli / genetics
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Gene Expression
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HIV Core Protein p24 / chemistry
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HIV Core Protein p24 / genetics
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HIV Core Protein p24 / isolation & purification
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Hepatitis C Antigens / chemistry
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Hepatitis C Antigens / genetics
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Hepatitis C Antigens / isolation & purification
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Hepatitis delta Antigens / chemistry
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Hepatitis delta Antigens / genetics
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Hepatitis delta Antigens / isolation & purification
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Humans
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In Vitro Techniques
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Isoelectric Point
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Recombinant Proteins / chemistry
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Recombinant Proteins / isolation & purification*
Substances
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Antibodies, Viral
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Antigens
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HIV Core Protein p24
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Hepatitis C Antigens
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Hepatitis delta Antigens
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Recombinant Proteins