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    J Chromatogr. 1992 Dec 23;584(2):256-60.

    High-performance liquid chromatography-electrochemical detection of 3-methylhistidine in human urine.

    Betto P, Ricciarello G, Pichini S, Dello Strologo L, Rizzoni G.

    Source

    Istituto Superiore di Sanità, Rome, Italy.

    Abstract

    A reversed-phase high-performance liquid chromatographic method is described for the determination of 3-methylhistidine content in human urine using pre-column derivatization with phenylisothiocyanate, isocratic elution with 15 mM sodium acetate-acetonitrile (92:8, v/v) and electrochemical detection. The limit of quantitation was 0.1 pmol. The method has been applied in routine analyses of 3-methylhistidine in both clinical and research work.

    PMID:
    1484110
    [PubMed - indexed for MEDLINE]

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